Table 2

The effects of trehalose concentration on the activity (initial rates) of the α-chymotrypsin precipitated by n-propanol in octane. The experiments were carried out in triplicate. The percentage error in a set was within 7 percent.
Starting trehalose (percent wv-1)
 percent trehalose precipitated
 Enzyme loadinga (weight percentage)
 Initial rateb (nmoles mg-1min-1)
 Visual morphology of enzyme precipitate
 Precipitation of trehalose in propanol (control)
0
 0
 -
 16.6
 Powder (EPRP)
 No precipitation
2
 65
 38.9
 40.0
 Powder (EPRP)
 No precipitation
5
 90
 15.5
 45.2
 Powder (EPRP)
 No precipitation
10
 94
 7.5
 63.2
 Powder-crystalline
 No precipitation
15
 88
 5.9
 120.0
 Crystalline
 Precipitation
20
 85
 4.6
 125.1
 Crystalline
 Precipitation
25
 78
 4.0
 143.2
 Crystalline
 Precipitation
30
 76
 3.5
 150.5
 Crystalline
 Precipitation
35
 72
 3.1
 152.0
 Crystalline
 Precipitation
40
 70
 2.8
 151.2
 Crystalline
 Precipitation
45
 65
 2.3
 96.4
 Crystalline
 Precipitation
Saturated trehalose solution
 60
 1.5
 87.4
 Crystalline
 Precipitation
PCMC (K2SO4)
 -
 5.8
 132.5
 Crystalline
 Precipitation

a Math

The amount of protein precipitated in each case was 0.83 mg.

bInitial rates were calculated from the aliquots taken over 10 to 60 min. The percentage conversions during these time periods were 1–30 percent and in a linear range.

Solanki and Gupta Chemistry Central Journal 2008 2:2   doi:10.1186/1752-153X-2-2